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ELISA Full Form in Medical-Enzyme-Linked Immunosorbent Assay

In a medical context, “ELISA” stands for “Enzyme-Linked Immunosorbent Assay.” ELISA is a widely used laboratory technique that helps detect the presence of specific proteins, antibodies, or antigens in a sample. It plays a crucial role in diagnosing various medical conditions, including infectious diseases (such as HIV and hepatitis), autoimmune disorders, allergies, and certain cancers.

Here’s how the ELISA process typically works:

  1. Coating: The wells of a microplate are coated with an antigen or antibody that is known to react with the target substance of interest.
  2. Incubation: The sample containing the unknown substance is added to the wells. If the substance is present, it will bind to the coated antigen or antibody.
  3. Washing: The microplate is washed to remove any unbound substances.
  4. Enzyme Conjugation: An enzyme-linked antibody is added to the wells. This enzyme will bind to any target substance that has attached to the coated antigen or antibody.
  5. Color Reaction: A substrate containing a color-changing enzyme substrate is added. If the target substance is present, an enzymatic reaction occurs, resulting in a color change.
  6. Measurement: The degree of color change is measured using a spectrophotometer, and this measurement provides information about the presence and quantity of the target substance.

ELISA is known for its sensitivity and specificity, making it a valuable tool in medical diagnostics and research. It is utilized in various healthcare settings, including clinical laboratories and research institutions, to detect and quantify specific molecules of interest, aiding in disease diagnosis and monitoring patient responses to treatment.

Some other possible full forms for ELISA.

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  4. European Laboratory for Intelligent Techniques in Speech and Audio Processing